Over-expression of UDP-glucose pyrophosphorylase in hybrid poplar affects carbon allocation.
Identifieur interne : 003B09 ( Main/Exploration ); précédent : 003B08; suivant : 003B10Over-expression of UDP-glucose pyrophosphorylase in hybrid poplar affects carbon allocation.
Auteurs : Heather D. Coleman [Canada] ; Thomas Canam ; Kyu-Young Kang ; David D. Ellis ; Shawn D. MansfieldSource :
- Journal of experimental botany [ 1460-2431 ] ; 2007.
Descripteurs français
- KwdFr :
- Amidon (métabolisme), Arbres (croissance et développement), Arbres (enzymologie), Arbres (physiologie), Carbone (métabolisme), Gluconacetobacter xylinus (génétique), Métabolisme glucidique (physiologie), Paroi cellulaire (métabolisme), Populus (croissance et développement), Populus (enzymologie), Populus (physiologie), Régions promotrices (génétique) (MeSH), Transcription génétique (MeSH), Transformation génétique (MeSH), UTP glucose 1-phosphate uridylyltransferase (métabolisme).
- MESH :
- croissance et développement : Arbres, Populus.
- enzymologie : Arbres, Populus.
- génétique : Gluconacetobacter xylinus.
- métabolisme : Amidon, Carbone, Paroi cellulaire, UTP glucose 1-phosphate uridylyltransferase.
- physiologie : Arbres, Métabolisme glucidique, Populus.
- Régions promotrices (génétique), Transcription génétique, Transformation génétique.
English descriptors
- KwdEn :
- Carbohydrate Metabolism (physiology), Carbon (metabolism), Cell Wall (metabolism), Gluconacetobacter xylinus (genetics), Populus (enzymology), Populus (growth & development), Populus (physiology), Promoter Regions, Genetic (MeSH), Starch (metabolism), Transcription, Genetic (MeSH), Transformation, Genetic (MeSH), Trees (enzymology), Trees (growth & development), Trees (physiology), UTP-Glucose-1-Phosphate Uridylyltransferase (metabolism).
- MESH :
- chemical , metabolism : Carbon, Starch, UTP-Glucose-1-Phosphate Uridylyltransferase.
- enzymology : Populus, Trees.
- genetics : Gluconacetobacter xylinus.
- growth & development : Populus, Trees.
- metabolism : Cell Wall.
- physiology : Carbohydrate Metabolism, Populus, Trees.
- Promoter Regions, Genetic, Transcription, Genetic, Transformation, Genetic.
Abstract
The effects of the over-expression of the Acetobacter xylinum UDP-glucose pyrophosphorylase (UGPase) under the control of the tandem repeat Cauliflower Mosaic Virus promoter (2x35S) on plant metabolism and growth were investigated in hybrid poplar (Populus albaxgrandidentata). Transcript levels, enzyme activity, growth parameters, leaf morphology, structural and soluble carbohydrates, and soluble metabolite levels were quantified in both transgenic and wild-type trees. Transgenic 2x35S::UGPase poplar showed impaired growth rates, displaying reduced height growth and stem diameter. Morphologically, 2x35S::UGPase trees had elongated axial shoots, and leaves that were substantially smaller in size when compared with wild-type trees at equivalent developmental stages. Biochemical analysis revealed significant increases in soluble sugar, starch, and cellulose contents, and concurrent decreases in lignin content. Lignin monomer composition was altered in favour of syringyl moieties. Detailed soluble metabolite analysis revealed that 2x35S::UGPase trees had as much as a 270-fold increase in the salicylic acid 2-O-beta-D-glucoside (SAG), a compound typically associated with the stress response. These data suggest that while it is possible to alter the allocation of carbon in favour of cellulose biosynthesis, whole plant changes result in unexpected decreases in growth and an increase in defence metabolites.
DOI: 10.1093/jxb/erm287
PubMed: 18182429
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Coleman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Wood Science, University of British Columbia, Vancouver, BC, V6T 1Z4 Canada.</nlm:affiliation><orgName type="university">Université de la Colombie-Britannique</orgName><country>Canada</country><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Canam, Thomas" sort="Canam, Thomas" uniqKey="Canam T" first="Thomas" last="Canam">Thomas Canam</name></author><author><name sortKey="Kang, Kyu Young" sort="Kang, Kyu Young" uniqKey="Kang K" first="Kyu-Young" last="Kang">Kyu-Young Kang</name></author><author><name sortKey="Ellis, David D" sort="Ellis, David D" uniqKey="Ellis D" first="David D" last="Ellis">David D. Ellis</name></author><author><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. 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Coleman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Wood Science, University of British Columbia, Vancouver, BC, V6T 1Z4 Canada.</nlm:affiliation><orgName type="university">Université de la Colombie-Britannique</orgName><country>Canada</country><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Canam, Thomas" sort="Canam, Thomas" uniqKey="Canam T" first="Thomas" last="Canam">Thomas Canam</name></author><author><name sortKey="Kang, Kyu Young" sort="Kang, Kyu Young" uniqKey="Kang K" first="Kyu-Young" last="Kang">Kyu-Young Kang</name></author><author><name sortKey="Ellis, David D" sort="Ellis, David D" uniqKey="Ellis D" first="David D" last="Ellis">David D. Ellis</name></author><author><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbohydrate Metabolism (physiology)</term><term>Carbon (metabolism)</term><term>Cell Wall (metabolism)</term><term>Gluconacetobacter xylinus (genetics)</term><term>Populus (enzymology)</term><term>Populus (growth & development)</term><term>Populus (physiology)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Starch (metabolism)</term><term>Transcription, Genetic (MeSH)</term><term>Transformation, Genetic (MeSH)</term><term>Trees (enzymology)</term><term>Trees (growth & development)</term><term>Trees (physiology)</term><term>UTP-Glucose-1-Phosphate Uridylyltransferase (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amidon (métabolisme)</term><term>Arbres (croissance et développement)</term><term>Arbres (enzymologie)</term><term>Arbres (physiologie)</term><term>Carbone (métabolisme)</term><term>Gluconacetobacter xylinus (génétique)</term><term>Métabolisme glucidique (physiologie)</term><term>Paroi cellulaire (métabolisme)</term><term>Populus (croissance et développement)</term><term>Populus (enzymologie)</term><term>Populus (physiologie)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Transcription génétique (MeSH)</term><term>Transformation génétique (MeSH)</term><term>UTP glucose 1-phosphate uridylyltransferase (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon</term><term>Starch</term><term>UTP-Glucose-1-Phosphate Uridylyltransferase</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arbres</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arbres</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gluconacetobacter xylinus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Gluconacetobacter xylinus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Amidon</term><term>Carbone</term><term>Paroi cellulaire</term><term>UTP glucose 1-phosphate uridylyltransferase</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Arbres</term><term>Métabolisme glucidique</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Carbohydrate Metabolism</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Promoter Regions, Genetic</term><term>Transcription, Genetic</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Régions promotrices (génétique)</term><term>Transcription génétique</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effects of the over-expression of the Acetobacter xylinum UDP-glucose pyrophosphorylase (UGPase) under the control of the tandem repeat Cauliflower Mosaic Virus promoter (2x35S) on plant metabolism and growth were investigated in hybrid poplar (Populus albaxgrandidentata). Transcript levels, enzyme activity, growth parameters, leaf morphology, structural and soluble carbohydrates, and soluble metabolite levels were quantified in both transgenic and wild-type trees. Transgenic 2x35S::UGPase poplar showed impaired growth rates, displaying reduced height growth and stem diameter. Morphologically, 2x35S::UGPase trees had elongated axial shoots, and leaves that were substantially smaller in size when compared with wild-type trees at equivalent developmental stages. Biochemical analysis revealed significant increases in soluble sugar, starch, and cellulose contents, and concurrent decreases in lignin content. Lignin monomer composition was altered in favour of syringyl moieties. Detailed soluble metabolite analysis revealed that 2x35S::UGPase trees had as much as a 270-fold increase in the salicylic acid 2-O-beta-D-glucoside (SAG), a compound typically associated with the stress response. These data suggest that while it is possible to alter the allocation of carbon in favour of cellulose biosynthesis, whole plant changes result in unexpected decreases in growth and an increase in defence metabolites.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18182429</PMID><DateCompleted><Year>2008</Year><Month>04</Month><Day>28</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>58</Volume><Issue>15-16</Issue><PubDate><Year>2007</Year></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>Over-expression of UDP-glucose pyrophosphorylase in hybrid poplar affects carbon allocation.</ArticleTitle><Pagination><MedlinePgn>4257-68</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/erm287</ELocationID><Abstract><AbstractText>The effects of the over-expression of the Acetobacter xylinum UDP-glucose pyrophosphorylase (UGPase) under the control of the tandem repeat Cauliflower Mosaic Virus promoter (2x35S) on plant metabolism and growth were investigated in hybrid poplar (Populus albaxgrandidentata). Transcript levels, enzyme activity, growth parameters, leaf morphology, structural and soluble carbohydrates, and soluble metabolite levels were quantified in both transgenic and wild-type trees. Transgenic 2x35S::UGPase poplar showed impaired growth rates, displaying reduced height growth and stem diameter. Morphologically, 2x35S::UGPase trees had elongated axial shoots, and leaves that were substantially smaller in size when compared with wild-type trees at equivalent developmental stages. Biochemical analysis revealed significant increases in soluble sugar, starch, and cellulose contents, and concurrent decreases in lignin content. Lignin monomer composition was altered in favour of syringyl moieties. Detailed soluble metabolite analysis revealed that 2x35S::UGPase trees had as much as a 270-fold increase in the salicylic acid 2-O-beta-D-glucoside (SAG), a compound typically associated with the stress response. These data suggest that while it is possible to alter the allocation of carbon in favour of cellulose biosynthesis, whole plant changes result in unexpected decreases in growth and an increase in defence metabolites.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Coleman</LastName><ForeName>Heather D</ForeName><Initials>HD</Initials><AffiliationInfo><Affiliation>Department of Wood Science, University of British Columbia, Vancouver, BC, V6T 1Z4 Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Canam</LastName><ForeName>Thomas</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Kyu-Young</ForeName><Initials>KY</Initials></Author><Author ValidYN="Y"><LastName>Ellis</LastName><ForeName>David D</ForeName><Initials>DD</Initials></Author><Author ValidYN="Y"><LastName>Mansfield</LastName><ForeName>Shawn D</ForeName><Initials>SD</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-25-8</RegistryNumber><NameOfSubstance UI="D013213">Starch</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.7.9</RegistryNumber><NameOfSubstance UI="D005957">UTP-Glucose-1-Phosphate Uridylyltransferase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D041822" MajorTopicYN="N">Gluconacetobacter xylinus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013213" MajorTopicYN="N">Starch</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005957" MajorTopicYN="N">UTP-Glucose-1-Phosphate Uridylyltransferase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>1</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>4</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>1</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18182429</ArticleId><ArticleId IdType="pii">erm287</ArticleId><ArticleId IdType="doi">10.1093/jxb/erm287</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Canam, Thomas" sort="Canam, Thomas" uniqKey="Canam T" first="Thomas" last="Canam">Thomas Canam</name><name sortKey="Ellis, David D" sort="Ellis, David D" uniqKey="Ellis D" first="David D" last="Ellis">David D. Ellis</name><name sortKey="Kang, Kyu Young" sort="Kang, Kyu Young" uniqKey="Kang K" first="Kyu-Young" last="Kang">Kyu-Young Kang</name><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name></noCountry><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Coleman, Heather D" sort="Coleman, Heather D" uniqKey="Coleman H" first="Heather D" last="Coleman">Heather D. Coleman</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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